rabbit polyclonal antibodies against rhbg (MedChemExpress)
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Rabbit Polyclonal Antibodies Against Rhbg, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+antibodies+against+rhbg/Aviptadil/pmc12108939-114-35-46
Average 90 stars, based on 1 article reviews
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1) Product Images from "HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions"
Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions
Journal: Biology
doi: 10.3390/biology14050498
Figure Legend Snippet: Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
Techniques Used: Expressing
Figure Legend Snippet: Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.
Techniques Used: Expressing
Figure Legend Snippet: ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.
Techniques Used: Sequencing, Luciferase, Reporter Assay
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Immunofluorescence:Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions. Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 ◦C with Incubation:Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions. Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 ◦C with |
