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rabbit polyclonal antibodies against rhbg  (MedChemExpress)


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    Structured Review

    MedChemExpress rabbit polyclonal antibodies against rhbg
    Changes in expression levels of HIF1A and <t>RHBG</t> in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
    Rabbit Polyclonal Antibodies Against Rhbg, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibodies+against+rhbg/Aviptadil/pmc12108939-114-35-46
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibodies against rhbg - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions"

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

    Journal: Biology

    doi: 10.3390/biology14050498

    Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
    Figure Legend Snippet: Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).

    Techniques Used: Expressing

    Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.
    Figure Legend Snippet: Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.

    Techniques Used: Expressing

    ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.
    Figure Legend Snippet: ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.

    Techniques Used: Sequencing, Luciferase, Reporter Assay

    Related Articles

    Immunofluorescence:

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions
    Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200). ..

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions.
    Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 ◦C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200). ..

    Incubation:

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions
    Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200). ..

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions.
    Article Snippet: Sections with a thickness of 10 μm were prepared using a freezing microtome (Leica CM3050 S, Solms, Wentzler, Germany). .. For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 ◦C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200). ..



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    Changes in expression levels of HIF1A and <t>RHBG</t> in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
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    Changes in expression levels of HIF1A and <t>RHBG</t> in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
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    Changes in expression levels of HIF1A and <t>RHBG</t> in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).
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    Image Search Results


    Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).

    Journal: Biology

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

    doi: 10.3390/biology14050498

    Figure Lengend Snippet: Changes in expression levels of HIF1A and RHBG in gills of Amur ide under 50 mM alkalinity treatment. * p < 0.05, *** 0.0001 < p < 0.001, **** p < 0.0001. ( A ) Relative mRNA expression levels of Hif1α after 7-day alkaline treatment; ( B ) Protein expression levels of HIF1A, RHBG, and ACTB under alkaline treatment of 0d and 3d; ( C ) Relative protein expression levels of RHBG under alkaline treatment of 0d and 3d; ( D ) Relative protein expression levels of HIF1A under alkaline treatment of 0d and 3d. Data are expressed as means ± SD ( n = 3).

    Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

    Techniques: Expressing

    Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.

    Journal: Biology

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

    doi: 10.3390/biology14050498

    Figure Lengend Snippet: Expression of HIF1A and RHBG in the gills of Amur ide at 0d and 3d after treatment with 50 mM alkalinity. Scale bars are 100 μm.

    Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

    Techniques: Expressing

    ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.

    Journal: Biology

    Article Title: HIF1A Regulates Rhbg Expression to Enhance Ammonia Excretion in Amur Ide ( Leuciscus waleckii ) Under Extreme Alkaline Conditions

    doi: 10.3390/biology14050498

    Figure Lengend Snippet: ( A ) The motif sequence of Hif1α obtained from the JASPAR database; ( B ) Dual-luciferase reporter assay verifying that HIF1A can bind to the Rhbg promoter. Data are expressed as means ± SD ( n = 3). The asterisk (***) denotes a significant difference.

    Article Snippet: For immunofluorescence experiments, the slides were washed with 1 × PBS for 20 min and blocked with 5% BSA at room temperature for 1 h. Thereafter, the sections were incubated overnight at 4 °C with rabbit polyclonal antibodies against RHBG of the Amur ide and HIF1A (MCE, Kenilworth, NJ, USA, diluted 1:200).

    Techniques: Sequencing, Luciferase, Reporter Assay

    Dendrograms of (a) Rhesus blood group-associated glycoprotein (Rhag/RhAG), (b) Rhesus family B glycoprotein (Rhbg/RhBG) and (c) Rhesus family C glycoprotein (Rhcg/RhCG) including those of Protopterus annectens . Numbers presented at each branch point represent bootstrap values from 100 replicates. Ciona intestinalis Rhag and Rhbg and Thelohanellus kitauei Rhcg are used as outgroups for their respective dendrogram.

    Journal: PLoS ONE

    Article Title: Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens , and effects of aestivation on their mRNA expression levels and protein abundance

    doi: 10.1371/journal.pone.0185814

    Figure Lengend Snippet: Dendrograms of (a) Rhesus blood group-associated glycoprotein (Rhag/RhAG), (b) Rhesus family B glycoprotein (Rhbg/RhBG) and (c) Rhesus family C glycoprotein (Rhcg/RhCG) including those of Protopterus annectens . Numbers presented at each branch point represent bootstrap values from 100 replicates. Ciona intestinalis Rhag and Rhbg and Thelohanellus kitauei Rhcg are used as outgroups for their respective dendrogram.

    Article Snippet: A commercial firm (GenScript, Piscataway, NJ, USA) was engaged to raise a rabbit polyclonal antibody against aa 198‒211 (CYRSGLRNGHDNEGS) of Rhag, a rabbit polyclonal antibody against aa 30‒43 (FVRYDKETDPKEWH) of Rhbg and a rabbit polyclonal antibody against aa 29‒42 (IRYSDEADAARWPE) of Rhcg from the gills of P . annectens .

    Techniques:

    mRNA expression of (a) rhesus blood group-associated glycoprotein ( rhag ), (b) rhesus family B glycoprotein ( rhbg ) and (c) rhesus family C glycoprotein ( rhcg ) in various tissues/organs of Protopterus annectens . The mRNA expression of rhag , rhbg and rhcg were examined in the gills (Gi), eyes (E), brain (B), heart (H), liver (Li), spleen (Sp), pancreas (P), gut (Gu), kidney (K), Lung (Lu), muscle (M) and skin (Sk) of P . annectens kept in fresh water.

    Journal: PLoS ONE

    Article Title: Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens , and effects of aestivation on their mRNA expression levels and protein abundance

    doi: 10.1371/journal.pone.0185814

    Figure Lengend Snippet: mRNA expression of (a) rhesus blood group-associated glycoprotein ( rhag ), (b) rhesus family B glycoprotein ( rhbg ) and (c) rhesus family C glycoprotein ( rhcg ) in various tissues/organs of Protopterus annectens . The mRNA expression of rhag , rhbg and rhcg were examined in the gills (Gi), eyes (E), brain (B), heart (H), liver (Li), spleen (Sp), pancreas (P), gut (Gu), kidney (K), Lung (Lu), muscle (M) and skin (Sk) of P . annectens kept in fresh water.

    Article Snippet: A commercial firm (GenScript, Piscataway, NJ, USA) was engaged to raise a rabbit polyclonal antibody against aa 198‒211 (CYRSGLRNGHDNEGS) of Rhag, a rabbit polyclonal antibody against aa 30‒43 (FVRYDKETDPKEWH) of Rhbg and a rabbit polyclonal antibody against aa 29‒42 (IRYSDEADAARWPE) of Rhcg from the gills of P . annectens .

    Techniques: Expressing

    Relative mRNA expression (determined using qPCR and calculated based on 2 -ΔΔCT ) of (a) rhesus blood group-associated glycoprotein ( rhag ), (b) rhesus family B glycoprotein ( rhbg ) and (c) rhesus family C glycoprotein ( rhcg ) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), after 6 days (d; the induction phase) or 6 months (mon; the maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar; the arousal phase) from 6 mon of aestivation. Results represent means ± S.E.M ( N = 5). Means not sharing the same letter are significantly different ( P < 0.05).

    Journal: PLoS ONE

    Article Title: Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens , and effects of aestivation on their mRNA expression levels and protein abundance

    doi: 10.1371/journal.pone.0185814

    Figure Lengend Snippet: Relative mRNA expression (determined using qPCR and calculated based on 2 -ΔΔCT ) of (a) rhesus blood group-associated glycoprotein ( rhag ), (b) rhesus family B glycoprotein ( rhbg ) and (c) rhesus family C glycoprotein ( rhcg ) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), after 6 days (d; the induction phase) or 6 months (mon; the maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar; the arousal phase) from 6 mon of aestivation. Results represent means ± S.E.M ( N = 5). Means not sharing the same letter are significantly different ( P < 0.05).

    Article Snippet: A commercial firm (GenScript, Piscataway, NJ, USA) was engaged to raise a rabbit polyclonal antibody against aa 198‒211 (CYRSGLRNGHDNEGS) of Rhag, a rabbit polyclonal antibody against aa 30‒43 (FVRYDKETDPKEWH) of Rhbg and a rabbit polyclonal antibody against aa 29‒42 (IRYSDEADAARWPE) of Rhcg from the gills of P . annectens .

    Techniques: Expressing, Control

    Immunoblots incubated with (a) anti-Rhesus blood group-associated glycoprotein (Rhag), (b) anti-Rhesus family B glycoprotein (Rhbg) and (c) anti-Rhesus family C glycoprotein (Rhcg) antibodies. Recombinant proteins of Rhag, Rhbg, Rhcg and the protein from the gills of Protopterus annectens kept in fresh water on day 0 (FW) were loaded for gel separation.

    Journal: PLoS ONE

    Article Title: Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens , and effects of aestivation on their mRNA expression levels and protein abundance

    doi: 10.1371/journal.pone.0185814

    Figure Lengend Snippet: Immunoblots incubated with (a) anti-Rhesus blood group-associated glycoprotein (Rhag), (b) anti-Rhesus family B glycoprotein (Rhbg) and (c) anti-Rhesus family C glycoprotein (Rhcg) antibodies. Recombinant proteins of Rhag, Rhbg, Rhcg and the protein from the gills of Protopterus annectens kept in fresh water on day 0 (FW) were loaded for gel separation.

    Article Snippet: A commercial firm (GenScript, Piscataway, NJ, USA) was engaged to raise a rabbit polyclonal antibody against aa 198‒211 (CYRSGLRNGHDNEGS) of Rhag, a rabbit polyclonal antibody against aa 30‒43 (FVRYDKETDPKEWH) of Rhbg and a rabbit polyclonal antibody against aa 29‒42 (IRYSDEADAARWPE) of Rhcg from the gills of P . annectens .

    Techniques: Western Blot, Incubation, Recombinant

    Protein abundance of (a) Rhesus blood group-associated glycoprotein (Rhag), (b) Rhesus family B glycoprotein (Rhbg) and (c) Rhesus family C glycoprotein (Rhcg) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), or after 6 days (d; the induction phase) or 6 months (mon; the maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar; the arousal phase) from 6 mon of aestivation. (i) Examples of immunoblot of Rhag, Rhbg or Rhcg. (ii) The protein abundance of Rhag, Rhbg or Rhcg expressed as arbitrary densitometric units per 100 μg, 100 μg or 50 μg protein, respectively. Results represent mean ± S.E.M. ( N = 4). Means not sharing the same letter are significantly different ( P <0.05).

    Journal: PLoS ONE

    Article Title: Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens , and effects of aestivation on their mRNA expression levels and protein abundance

    doi: 10.1371/journal.pone.0185814

    Figure Lengend Snippet: Protein abundance of (a) Rhesus blood group-associated glycoprotein (Rhag), (b) Rhesus family B glycoprotein (Rhbg) and (c) Rhesus family C glycoprotein (Rhcg) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), or after 6 days (d; the induction phase) or 6 months (mon; the maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar; the arousal phase) from 6 mon of aestivation. (i) Examples of immunoblot of Rhag, Rhbg or Rhcg. (ii) The protein abundance of Rhag, Rhbg or Rhcg expressed as arbitrary densitometric units per 100 μg, 100 μg or 50 μg protein, respectively. Results represent mean ± S.E.M. ( N = 4). Means not sharing the same letter are significantly different ( P <0.05).

    Article Snippet: A commercial firm (GenScript, Piscataway, NJ, USA) was engaged to raise a rabbit polyclonal antibody against aa 198‒211 (CYRSGLRNGHDNEGS) of Rhag, a rabbit polyclonal antibody against aa 30‒43 (FVRYDKETDPKEWH) of Rhbg and a rabbit polyclonal antibody against aa 29‒42 (IRYSDEADAARWPE) of Rhcg from the gills of P . annectens .

    Techniques: Quantitative Proteomics, Control, Western Blot